Experimental oral chronic infection induced by actinomyces israelii and propionibacterium acnes
Abstract
The purpose of this study was to evaluate an experimental animal model of oral chronic infection induced by Actinomyces israelii and Propionibacterium acnes in mice. Swiss/NIH mice (n=100), 21 days of age, male and female were divided into two groups of 45 animals. A. israelii (n=45) and P. acnes (n=45) were inoculated in the anterior mandibular paraperiosteal periodontal tissue associated with sodium alginate gel particles. The animals were evaluated clinically and microscopically at 1, 3, 7, 15, 21, 30, and 45 days after inoculation. Actinomycotic and propioni lesions were induced in all animals. In control mice (n=10), no lesions were noted; however, differences in the clinical and histopathological evolutions of actinomycosis and propioni lesions were observed and are discussed in this study. Microorganisms entrapped in alginate gel provided a prolonged bacterial irritation, and chronic histopathologic features similar to those seen in human actinomycosis could be detected.
Downloads
References
Eady EA, Ingham E. Propionibacterium acnes: friend or foe? Rev Med Microbiol 1994; 5:163-173.
Alamillos-Granados FJ, Dean-Ferrer A, García- López A, López-Rubio F. Actinomycotic ulcer of the oral mucosa: an ununsual presentation of oral actinomycosis. Br J Oral Maxillofac Surg 2000; 38:121-123.
Pulverer G, Schutt- Gerowitt H, Schaal KP. Human cervicofacial actinomycosis: microbial data for 1997 cases. Clin Infect Dis 2003; 37:490-497.
Emmanuelli JL. Infections granulomatous diseases of the head and neck. Am J Otolaryngol 1993; 14:155-167.
O’Grady JF, Reade PC. Periapical actinomycosis involving Actinomyces israelii. J Endod 1988; 14: 147-149.
Miller M, Haddad AJ. Cervicofacial actinomycosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85:496-508.
Bourdeaut F, Quartier P, Alkaer G, Fisher A, Casanova JL, Blanche S. Propionibacterium acnes chest infections in patients with chronic granulomatous disease: case reports. Clin Infect Dis 2002; 34:853-854.
Howell A, Stephan RM, Paul F. Prevalence of Actinomyces israelii, A. naeslundii, Bacterionema matruchotii, and Candida albicans in selected ares of the oral cavity and saliva. J Dent Res 1962; 41:1050-1058.
Hoshino E. Predominant obligate anaerobes in human carious dentine. J Dent Res 1985; 64:1195-1198.
Kamma JJ, Nakou M, Gmur R, Baehi PC. Microbiological profile of early onset/ aggressive periodontitis patients. Oral Microbiol Immunol 2004; 19:314-321.
Shen S, Samaranayake LP, Yip HK. Coaggregation profiles on the microflora from root surface caries lesions. Arch Oral Biol 2005; 50:23-32.
Bak UB, Yamamoto H, Azuma R. Experimental actinomycotic abscess in mice of several strains. Nation Inst Anim Heal Quart 1972; 12:232-233.
Brown JR, von Lichtenberg F. Experimental actinomycosis in mice: study of pathogenesis. Arch Pathol 1970; 90:391-402.
Behbehani MJ, Jordan HV. Comparative pathogenicity of Actinomyces species in mice. J Med Microbiol 1982; 15:465-473.
Behbehani MJ, Heeley JD, Jordan HV. Comparative histopathology of lesions produced by Actinomyces israelii, Actinomyces naeslundii and Actinomyces viscosus in mice. Am J Pathol 1983; 110:267-274.
Asgor Moral MA, Ohshima H, Maeda T, Hoshino E. Experimental chronic infection induced in mice by Actinomyces israelii entrapped in alginate gel. Arch Oral Biol 1998; 43:485-96.
Sumita M, Iwaku M, Hoshino E. Experimental actinomycosis in mice using alginate gel particles containing Actinomyces israelii. Endod Dent Traumatol 1998; 14:137-143.
Luna LG. Methods for bacteria, fungi, and inclusion bodies. In: Manual of histologic staining Methods of the Armed Forces Institute of Pathology, editors. New York: McGraw-Hill; 1968. p. 217-240.
Orstavik D, Mjör IA. Histopathology and x-ray microanalysis of the subcutaneous tissue response to endodontic sealers. J Endod 1988; 14:45-49.
National Committee for Clinical Laboratory Standards, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Fifth Edition:.M7-A5 NCCLS, Wayne, PA ; 2001.
Hillier SL, Moncla BJ. Peptostreptococcus, Propionibacterium, Eubacterium, and other Nonsporeforming anaerobic Gram-positive
Bacteria. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, editors. Manual of clinical microbiology. Washington: ASM Press;1995. p. 587-602.
Jordan HV, Kelly DM. Persistence of associated Gram-negative bacteria in experimental actinomycotic lesions in mice. Infect Immun 1983; 40:487-489.
Yu SM, Stashenko P. Identification of inflammatory cells in developing rat periapical lesions. J Endod 1987; 13:535-540.
Walton RE, Ardjmand K. Histological evaluation of the presence of bacteria in induced periapical lesions in monkeys. J Endod 1992; 18:216-221.
Tani-Ishi N, Wang CY, Tanner A, Stashenko P. Changes in root canal microbiota during the development of rat periapical lesions. Oral Microbiol Immunol 1994; 9:129-135. 26. Cintron JR, Pino AD, Duarte B, Wood D. Abdominal actinomycosis. Dis Colon Rectum 1996; 39:105-108.
Nagler R, Peled M, Laufer D. Cervicofacial actinomycosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997; 83:652-656.
Oppenheimer S, Millaer GS, Kenneth K, Blechman H. Periapical actinomycosis: an unusual case report. Oral Surg 1978; 46:101-106.
Nishimura RS. Periapical actinomycosis. J Endodontics 1986; 12:76-79.
Georg LK, Colemam, RM. Comparative pathogenicity of various Actinomyces species. In: Pauser H, editors: The Actinomycetales. Jena: Gustav Fischer; 1970. p. 35-45.
