Alkali soluble fluoride deposits on bovine dental enamelaftertreatmentwithamouthwashcontaining fluoride and chlorhexidine

Authors

  • Isabel Cristina Gavazzoni Bandeira de Andrade Faculdade São Leopoldo Mandic / Fundação Universitária Regional de Blumenau
  • Ilione Kruschewsky Costa Sousa Oliveira Faculdade São Leopoldo Mandic
  • Priscila Leite Fahel Guimarães Faculdade São Leopoldo Mandic
  • Bruna Krouwel Peres Faculdade São Leopoldo Mandic
  • Fabiana Mantovani Gomes França Faculdade São Leopoldo Mandic
  • Roberta Tarkany Basting Faculdade São Leopoldo Mandic
  • Ynara Bosco de Oliveira Lima-Arsati Universidade Estadual de Feira de Santana

Keywords:

Fluorides, Topical, Chlorhexidine, Mouthwash

Abstract

Aim: The aim of this in vitro study was to determine whether the addition of chlorhexidine (CHX) to a mouthwash containing fluoride (F) diminishes the concentration of soluble F and its reactivity with bovine dental enamel.

Materials and Methods: First, the concentration of F found in mouthwashs was determined by an ion-specific electrode. To test their reactivity, 30 slabs of bovine dental enamel (5 x 5mm) were distributed into three groups (n = 10), according to the treatment applied, for 30 seconds: control group (distilled deionized water); NaF group (0.05% sodium fluoride); NaF + CHX group (Noplak Max®: 0.05% sodium fluoride + 0.12% chlorhexidine digluconate). After, the slabs were washed with distilled deionized water and individually immersed in artificial saliva for 30 minutes. They were dried and then individually immersed in 0.5 mL of 1M KOH for 24 hours, under agitation. After buffering the samples with HCI containing TISAB II, the concentration of F present in the KOH solutions was determined be means of an ion-specific electrode.

Results: No significant difference in the concentration of soluble F in the studied mouthwashs (NaF and NaF+CHX; Student’s t-test, p > 0.05) could be observed. Furthermore, no difference could be identified among the alkali soluble fluoride deposits from the NaF and NaF+CHX groups, and both groups presented higher deposit levels than did the control group (Kruskal-Wallis and Dunn´s method, p < 0.05).

Conclusion: The addition of CHX to a mouthwash containing F did not decrease its soluble F concentration, nor its reactivity to bovine dental enamel.

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References

Brasil. Ministério da Saúde. Secretaria de Atenção à Saúde. Ce EIF, Fejerskov O. Chemical interactions between the tooth and oral fluids. In: Fejerskov O, Kidd E. Dental caries. The disease and its clinical management. 2nd ed. Oxford: Blackwell Munksgaard; 2008. chap. 12.

Cury JA, Tenuta LM. Enamel remineralization: controlling the caries disease or treating early caries lesions? Braz Oral Res. 2009; 23 Suppl 1:23-30.

Ten Cate JM, Larsen MJ, Pearce EIF, Fejerskov O. Chemical interactions between the tooth and oral fluids. In: Fejerskov O, Kidd E. Dental caries. The disease and its clinical management. 2nd ed. Oxford: Blackwell Munksgaard; 2008. chap. 12.

Saxegaard E, Rölla G. Fluoride acquisition on and in human enamel during topical application in vitro. Scand J Dent Res. 1988; 96:523-35.

Armitage GC. Biological bases for periodontal therapy. 2nd ed. São Paulo: Santos; 1993.

Schiott CR. Effect of chlorhexidine on the microflora of the oral cavity. J Periodontal Res. 1973; 12:7-10.

Scheie AA, Eggen KH, Rolla G. Glucosyltransferase activity in human in vivo formed enamel pellicle and in whole saliva. Scand J Dent Res. 1987; 95:212-5.

Van Rijkom HM, Truin GJ, Van‘t Hof MA. A meta-analysis of clinical studies on the cariesinhibiting effect of chlorhexidine treatment. J Dent Res. 1996; 75:790-5.

Emilson CG, Krasse B, Westergren G. Effect of a fluoride-containing chlorhexidine gel on bacteria in human plaque. Scand J Dent Res. 1976; 84: 56-62.

McDermid AS, Marsh PD, Keevil CW, Ellwood DC. Additive inhibitory effects of combinations Andrade et al. Reactivity of a fluoride-chlorhexidine containing mouthwash of fluoride and chlorhexidine on acid production by Streptococcus mutans and Streptococcus sanguis. Caries Res. 1985; 19:64-71.

Meurman JH. Ultrastructure, growth and adherency of Streptococcus mutans after treatment with chlorhexidine and fluoride. Caries Res. 1988; 22:283-7.

Luoma HH, Murtomaa H, Nuuja T, Nyman A, Nummikoski P, Ainamo J et al. A simultaneous reduction of caries and gingivitis in a group of schoolchildren receiving chlorhexidine-fluoride applications:results after 2 years. Caries Res. 1978; 12:290-8.

Katz S. The use of fluoride and chlorhexidine for the prevention of radiation caries. J Am Dent Assoc. 1982; 104:164-70.

Freitas CS, Diniz HFO, Gomes JB, Sinisterra RD, Cortés ME. Evaluation of the substantivity of chlorhexidine in association with sodium fluoride in vitro. Pesqui Odontol Bras. 2003; 17:78-81.

Barkvoll GR, Rölla G, Bellagamba S. Interaction between chlorhexidine digluconate and sodium monofluorophosphate in vitro. Scand J Dent Res. 1988; 96:30-3.

Del Bel Cury AA, Rebelo MAB, Cury JA. Efeito do bochecho com clorexidina (CH) e flúor (F) na redução de formação de placa dental e incorporação de flúor no esmalte dental. Rev Bras Odontol. 1994; 51: 26-9.

Serra MC, Cury JA. The in vitro effect of glass ionomer cement restoration on enamel subjected to demineralization and remineralization model. Quintessence Int. 1992; 23:143-7.

Caslavska V, Moreno EC, Brudevold F. Determination of the calcium fluoride formed from in vitro exposure of human enamel to fluoride solutions. Arch Oral Biol. 1975; 20:333- 9.

Featherstone JD. Delivery challenges for fluoride, chlorhexidine and xylitol. BMC Oral Health. 2006; 6:S8.

Tenuta LMA, Cury JA. Fluoride use in Dentistry and its anti-caries mechanism: part I. J Assoc Bras Odontol. 2008. [updated 2010 Jun 29; cited 2010 Sept 13]. Available at: http://www.abo.org.br/jornal/115/artigo1.php

Koo H. Strategies to enhance the biological effects of fluoride on dental biofilms. Adv Dent Res. 2008; 20:17-21.

Hayacibara MF, Leme AFP, Lima YBO, Gonçalves NCAV, Queiroz CS, Gomes MJ, Kozlowski F. Alkali-soluble fluoride deposition on enamel after professional application of topical fluoride in vitro. J Appl Oral Sci. 2004; 12:18-21.

Tabchoury CPM, Pierobon CN, Cury JA. Concentration and bioavailability of fluoride in mouthrinses prepared in dispensing pharmacies. J Appl Oral Sci. 2005; 13:41-6.

Arthur RA, Tabchoury CPM, Giancristófaro M, Del Bel Cury AA, Cury JA. Effect of preservatives on reactivity of fluoride with dental enamel. Rev Gaucha Odontol. 2007; 55:375-9.

Duarte AR, Peres MA, Vieira RS, Ramos-Jorge ML, Modesto A. Effectiveness of two mouth rinses solutions in arresting caries lesions: a short-term clinical trial. Oral Health Prev Dent. 2008; 6:231-8.

Poulsen S. Fluoride-containing gels, mouth rinses and varnishes: an update of evidence of efficacy. Eur Arch Paediatr Dent. 2009; 10:157-61.

Jenkins S, Addy M, Newcombe R. Evaluation of a mouthrinse containing chlorhexidine and fluoride as an adjunct to oral hygiene. J Clin Periodontol. 1993; 20:20-5.

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Published

2016-05-10

How to Cite

Andrade, I. C. G. B. de, Oliveira, I. K. C. S., Guimarães, P. L. F., Peres, B. K., França, F. M. G., Basting, R. T., & Lima-Arsati, Y. B. de O. (2016). Alkali soluble fluoride deposits on bovine dental enamelaftertreatmentwithamouthwashcontaining fluoride and chlorhexidine. Arquivos Em Odontologia, 47(2). Retrieved from https://periodicos.ufmg.br/index.php/arquivosemodontologia/article/view/3561

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