Antimicrobial effect of resveratrol on an etiological agent of human dental caries, Streptococcus mutans
DOI:
https://doi.org/10.35699/2178-1990.2024.47482Keywords:
microbiology, resveratrol, dental cariesAbstract
Aim: To evaluate the antimicrobial effect of resveratrol on the bacterium S. mutans.
Methods: In the execution of the study, samples were cultured on petri dishes plated with Mueller-Hinton Agar, containing sterile 6 mm diameter Blank discs (Cecton). The discs were soaked with 10 μl of negative control (phosphate-buffered saline - PBS), positive control (0.12% chlorhexidine), and different concentrations of resveratrol (100 μg/mL, 200 μg/mL, 400 μg/mL, and 800 μg/mL). The tests were performed in triplicate, totaling 18 analyses.
Results: After 24 hours of incubation, the halos were evaluated and measured using a digital caliper. The data collected were processed statistically using the Anova way program. The diameter measurement for the positive control halo was 2.84 cm; for the resveratrol at 800 μg/mL, it was 0.6 cm; at 400 μg/mL, it was 0.42 cm; and at 200 and 100 μg/mL, it was zero.
Conclusion: Resveratrol did not demonstrate efficiency against the bacteria when compared to the negative control (PBS). According to literature, resveratrol produces an antibacterial activity, although its optimal concentration for inhibitory effect is not yet well established.
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References
Petersen P, Ogawa H. Prevention of dental caries through the use of fluoride--the WHO approach. Community Dent Health. 2016;33(2):66-8. PMID: 27352461.
Albergaria L. Abordagem conservadora de lesões cariosas profundas: uma revisão de literatura. Rev Odontol Araçatuba. 2022;43(2):12-60.
Nascimento GP, Barbosa EV, Brum SC, Soares LC. Qualificação de Streptococcus mutans em amostras de saliva de pacientes com cavidades cariosas. Braz J Surg Clin Res. 2017;19(3):23-6.
Gouvêa CCM. Avaliação antibiofilme e anticariogênica de nanocompósitos formados por polifosfatos de sódio e flúor associados à nanopartículas de prata: estudo in vitro (Tese). Araçatuba: Faculdade de Odontologia, UNESP; 2018. 61 p.
Amaral LA, Tonini IGO, Medino IC, Ribas BA, Silva TKR, Tortorella CCS. Efeitos da suplementação de resveratrol na saúde humana: uma revisão integrativa. Visão Acad. 2018;19(3):74-9. doi: http://dx.doi.org/10.5380/acd.v19i3.59533.
Pandini J. Avaliação do efeito do resveratrol sobre os tecidos periodontais de ratos submetidos à periodontite experimental. Dissertação (Mestrado em odontologia). Cascavel: Universidade Estadual do Oeste do Pará; 2019.31 f.
Park E, Pezzuto J. The pharmacology of resveratrol in animals and humans. Biochim Biophys Acta. 2015;1852(6):1071-113. doi:10.1016/j.bbadis.2015.01.014
Ferreira S, Silva F, Queiroz JA, Oleastro M, Domingues FC. Resveratrol against Arcobacter butzleri and Arcobacter cryaerophilus: activity and effect on cellular functions. Int J Food Microbiol. 2014;180:62-8. doi:10.1016/j.ijfoodmicro.2014.04.004
Paulo L, Oleastro M, Gallardo E, Queiroz JA, Domingues F. Anti-Helicobacter pylori and urease inhibitory activities of resveratrol and red wine. Food Res Int. 2011;44(4):964-9. doi:10.1016/j.foodres.2011.02.017
Selma MV, Larrosa M, Beltrán D, Lucas R, Morales JC, Tomás-Barberán F. Resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives reduce Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes Scott A adhesion to colonic epithelial cell lines. J Agric Food Chem. 2012;60(30):7367-74. doi:10.1021/jf203967u
Brown JC, Jiang X. Activities of muscadine grape skin and polyphenolic constituents against Helicobacter pylori. J Appl Microbiol. 2013;114(4):982-91.
Taguri T, Tanaka T, Kouno I. Espectro antibacteriano de polifenóis e extratos vegetais dependendo da estrutura hidroxifenil. Biol Pharm Bull. 2006;29(11):2226-35.
Ma DSL, Tan LT-H, Chan K-G, Yap HY, Pusparajah P, Chuah L-H et al. Resveratrol - potencial agente antibacteriano contra patógenos de origem alimentar. Front Pharmacol. 2018;9:102.
Vestergaard M, Ingmer H. Antibacterial and antifungal properties of resveratrol. Int J Antimicrob Agents. 2019;53(6):716-23.
Paulo L, Ferreira S, Gallardo E, Queiroz JA, Domingues F. Antimicrobial activity and effects of resveratrol on human pathogenic bacteria. World J Microbiol Biotechnol. 2010;26:1533-8.
Chan MM-Y. Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem Pharmacol. 2002;63(2):99-104.
Docherty JJ, Fu MM, Tsai M. Resveratrol selectively inhibits Neisseria gonorrhoeae and Neisseria meningitidis. J Antimicrob Chemother. 2001;47(2):243-4.
Tegos G, Stermitz FR, Lomovskaya O, Lewis K. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrob Agents Chemother. 2002;46(10):3133-41.
Paulo L, Ferreira S, Gallardo E, Queiroz JA, Domingues F. (2010). Antimicrobial activity and effects of resveratrol on human pathogenic bacteria. World J Microbiol Biotechnol. 2010;26:1533-8. doi:10.1007/s11274-010-0325-7.
Pezzuto JM. Resveratrol: vinte anos de crescimento, desenvolvimento e polêmica. Biomol Ther (Seoul). 2019;27(1):1-14. doi: 10.4062/biomolther.2018.176.
Silva D. Streptococcus orais: perfil de resistência antimicrobiana. Tese (Mestrado em medicina dentária). Porto: Faculdade de medicina dentária, Universidade do Porto; 2018. 31 p.
Vestergaard M, Ingmer H. Antibacterial and antifungal properties of resveratrol. Int J Antimicrob Agents. 2019;53:716-23.
Malaguarnera L. Influence of resveratrol on the immune response. Nutrients. 2019:26;11(5):946. doi: 10.3390/nu11050946.
