Dental Students’ Knowledge of the Differential Diagnosis of Developmental Defects of Enamel (DDE)

a Comparative Cross-Sectional Study

Authors

DOI:

https://doi.org/10.35699/2178-1990.2025.59245

Keywords:

dental enamel hypoplasia, dental fluorosis, dental enamel, differential diagnosis, dental students

Abstract

Introduction: Developmental Defects of Enamel (DDE) are disturbances in enamel formation that can compromise tooth function and esthetics and increase susceptibility to dental caries. Accurate diagnosis is essential for appropriate clinical management. 

Aim: To compare the ability to perform differential diagnosis of DDE among undergraduate dental students in their penultimate (3rd year; G1) and final (4th year; G2) years of study at a private university. 

Methods: A total of 121 students participated in this comparative cross-sectional study (G1: n=71; G2: n=50). Data was collected through an online questionnaire comprising ten clinical case scenarios that required differential diagnosis of DDE. One point was awarded for each correct response, and the total scores were analyzed within and between groups. Statistical comparisons were performed using Chi-square, Fisher’s Exact test, and Mann-Whitney U test (α=5%). 

Results: The mean age of participants was 22.5 ± 4.0 years, with a predominance of females (82.6%). Integrated Dentistry for Children and Adolescents (71.9%) was the most frequently reported discipline addressing DDE. Regarding perceived preparedness, 39.7% of students reported that their coursework provided sufficient knowledge for DDE diagnosis. The overall mean score of correct diagnoses among all participants was 6.2 ± 1.8. Group G2 had a significantly higher mean score (6.6 ± 2.0) compared to G1 (6.0 ± 1.5) (p < 0.05). 

Conclusion: Dental students demonstrated a moderate level of diagnostic accuracy in identifying DDE. Final-year students demonstrated greater proficiency in the differential diagnosis of DDE than penultimate-year students, suggesting progressive knowledge acquisition throughout the dental curriculum. Nevertheless, diagnostic challenges remain, underscoring the need for ongoing education and reinforcement of this topic. 

References

Simmer JP, Hu JC. Dental enamel formation and its impact on clinical dentistry. J Dent Educ. 2001;65(9):896-905.

Lacruz RS, Habelitz S, Wright JT, Paine ML. Dental enamel formation and implications for oral health and disease. Physiol Reviews. 2017;97(3):939-93.

Alghadeer A, Hanson-Drury S, Patni AP, Ehnes DD, Zhao YT, Li Z, et al. Single-cell census of human tooth development enables generation of human enamel. Dev Cell. 2023;58(20):2163-80.e9.

Patel A, Aghababaie S, Parekh S. Hypomineralisation or hypoplasia? Br Dent J. 2019;227(8):683-6. Erratum in: Br Dent J. 2023; 235(12):937.

Collignon AM, Vergnes JN, Germa A, Azogui S, Breinig S, Hollande C, et al. Factors and mechanisms involved in acquired developmental defects of enamel: A scoping review. Front Pediatr. 2022;10:836708.

Nanci A. Enamel: Composition, formation, and structure. In: Nanci A, editor. Ten Cate’s oral histology: Development, structure, and function. 9th. ed. St. Louis, Missouri: Elsevier; 2018. p.288-369.

Manton DJ, Vieira AR. Chapter 4: Development defects of enamel and dentine and coronal caries. Monogr Oral Sci. 2023;31:37-49.

Seow WK. Developmental defects of enamel and dentine: challenges for basic science research and clinical management. Aust Dent J. 2014;59(Suppl 1):143-54.

Ramirez FLM, Cepeda MAAN, Palma GC, Lartigue CG, Rodriguez RL, Ramirez EL, et al. Developmental enamel defects: a review. Int J Appl Dent Sci. 2024;10(4):359-62.

Martignon S, Bartlett D, Manton DJ, Martinez-Mier EA, Splieth C, Avila V. Epidemiology of erosive tooth wear, dental fluorosis and molar incisor hypomineralization in the American continent. Caries Res. 2021;55(1):1-11.

Silva MJ, Mohandas N, Craig JM, Manton DJ, Saffery R, Southey MC, et al. DNA methylation in childhood dental caries and hypomineralization. J Dent. 2022;117:103913.

Sunderland EP, Smith CJ, Sunderland R. A histological study of the chronology of initial mineralization in the human deciduous dentition. Arch Oral Biol. 1987;32(3):167-74.

Zhang Y, Li H, Cui D, Liu Y, Tian Q, Zheng L, et al. Epigenetics in developmental defects of enamel: A scoping review. Oral Dis. 2023;29(6):2366-75.

FDI World Dental Federation. A review of the developmental defects of enamel index (DDE Index). Commission on Oral Health, Research & Epidemiology. Report of an FDI Working Group. Int Dent J. 1992;42(6):411-26.

Brook AH, Elcock C, Hallonsten AL, Poulsen S, Andreasen J, Koch G, et al. The development of a new index to measure enamel defects. In: Brook AH, ed. Dental Morphology. Sheffield: Academic Press, 2001; 59-66.

Corrêa-Faria P, Paixão-Gonçalves S, Ramos-Jorge ML, Paiva SM, Pordeus IA. Developmental enamel defects are associated with early childhood caries: Case- control study. Int J Paediatr Dent. 2020;30(1):11-7.

Costa FS, Silveira ER, Pinto GS, Nascimento GG, Thomson WM, Demarco FF. Developmental defects of enamel and dental caries in the primary dentition: A systematic review and meta-analysis. J Dent. 2017;60:1-7.

Castañeda-Sarmiento S, Uchima Koecklin KH, Barahona Hernandez MB, Santos GP, Bruno Luyo JC, Sánchez Sotomayor JC, et al. Association between developmental defects of enamel and early childhood caries in children under 6 years old: A systematic review and meta-analysis. Heliyon. 2022;8(9):e10479.

Salanitri S, Seow WK. Developmental enamel defects in the primary dentition: aetiology and clinical management. Aust Dent J. 2013;58(2):133-40; quiz 266.

Lins RBE, Andrade AKM, Duarte RM, Meireles SS. Influence of three treatment protocols for dental fluorosis in the enamel surface: an in vitro study. Rev Cient CRO- RJ (Online). 2019;4(1):79-86.

Gamboa GCS, Lee GHM, Ekambaram M, Yiu CKY. Knowledge, perceptions, and clinical experiences on molar incisor hypomineralization among dental care providers in Hong Kong. BMC Oral Health. 2018;18(1):217.

Delgado RM, Botelho J, Machado V, Mendes JJ, Lopes LB. Knowledge, perception, and clinical experiences on molar incisor hypomineralization amongst Portuguese dentists. BMC Oral Health. 2022;22(1):250.

Craveia J, Rouas P, Carat T, Manton DJ, Boileau MJ, Garot E. Knowledge and Management of First Permanent Molars with Enamel Hypomineralization among Dentists and Orthodontists. J Clin Pediatr Dent. 2020;44(1):20-7.

Costa Rosa T, Spinelli LR, Silva FMF, Costa MC, Neves AA. Perceptions, attitudes, and clinical experiences of Brazilian dental practitioners towards molar incisor hypomineralisation: a cross-sectional study. Eur Arch Paediatr Dent. 2024; 25(6):855-68.

Hamza B, Elhennawy K, van Waes H, Papageorgiou SN. Knowledge, attitudes, and beliefs regarding molar incisor hypomineralisation amongst Swiss dental students. BMC Oral Health. 2021;21(1):548.

Masri AABHA, Khang KKY, Shen LLW, Ekambaram M, Loch C. Knowledge of dental enamel defects amongst undergraduate dental students - a cross-sectional survey. Eur J Dent Educ. 2021;25(4):711-6.

Ferla JO, Rodrigues JA, Leonetti ES, Suguio K, Shibli JA, Cassoni A. Knowledge of dental fluorosis of undergraduate dental students at a private university in Brazil. N Am J Med Sci. 2010;2(8):371-5.

Aldaigy R, Alotaibi M, Alnowaiser D, Albahely R, Bachat R, Alshaya A, et al. Awareness of dental fluorosis among undergraduate dental students in Riyadh Region: a survey-based study. Saudi Dent J. 2019;31(Suppl):S74.

Ghanim A, Morgan M, Mariño R, Manton D, Bailey D. Perception of molar- incisor hypomineralisation (MIH) by Iraqi dental academics. Int J Paediatr Dent. 2011;21(4):261-70.

Tarazona-Valero V, Almerich-Silla JM, Iranzo-Cortés JE, Ortolá-Siscar JC, Almerich-Torres T. Knowledge and perception regarding molar incisor hypomineralisation among dental students and dental hygienist students in Spain: a cross-sectional study. BMC Oral Health. 2024;24(1):300.

Cagetti MG, Salerno C, Bontà G, Bisanti A, Maspero C, Tartaglia GM, et al. Dental and dental hygiene students’ knowledge and capacity to discriminate the developmental defects of enamel: A self-submitted questionnaire survey. Children (Basel). 2022;9(11):1759.

Martins TGO, Carvalho NO, Rocha CT, Neves BG. Knowledge and perception of Brazilian dental students about molar-incisor hypomineralization. Pesqui Bras Odontopediatria Clín Integr. 2025;25:e240065.

Barzotto I, Rigo L. Clinical decision making for diagnosis and treatment of dental enamel injuries. J Hum Growth Dev. 2018;28(2):189-98.

Seow WK. Clinical diagnosis of enamel defects: pitfalls and practical guidelines. Int Dent J. 1997;47(3):173-82.

Wang M, Liang S, Jiang T. Comparison of case-based and lecture-based learning in dental fluorosis diagnostic ability with visual analog scale assessment. BMC Med Educ. 2024; 24(1):761.

Sapir S, Shapira J. Clinical solutions for developmental defects of enamel and dentin in children. Pediatr Dent. 2007;29(4):330-6.

Foulds H. Developmental defects of enamel and caries in primary teeth. Evid Based Dent. 2017;18(3):72-3.

Ardelean LV, Rusu LC. Enamel and dentin-pulp complex. IntechOpen; 2024.

Alanzi A, Faridoun A, Kavvadia K, Ghanim A. Dentists’ perception, knowledge, and clinical management of molar-incisor-hypomineralisation in Kuwait: a cross- sectional study. BMC Oral Health. 2018;18(1):34.

Downloads

Published

2025-12-15

Issue

Section

Artigos

How to Cite

Dental Students’ Knowledge of the Differential Diagnosis of Developmental Defects of Enamel (DDE): a Comparative Cross-Sectional Study. (2025). Arquivos Em Odontologia, 61, 200-212. https://doi.org/10.35699/2178-1990.2025.59245