Silver nanoparticles in PMMA prosthesis bases to control microbial activity
DOI:
https://doi.org/10.35699/2178-1990.2021.26466Keywords:
Nanotechnology, Silver nitrate, Polymethyl methacrylate, Denture basesAbstract
Aim: To perform an in vitro evaluation of the activity of Staphylococcus aureus and Candida albicans in conventional prosthesis bases, based on methyl polymethylmethacrylate with silver nanoparticles incorporated into the composition.
Methods: An experimental laboratory study was carried out using commercially available self-curing acrylic resins, Vipi Flash/VIPI and JET/Clássico. Eighty specimens were manufactured and divided into 16 groups (n = 5), referent to the resin brand, treatment (incorporation and immersion in the silver nanoparticle solution), and inoculated microorganism. The nanoparticles were synthesized with polymethacrylic acid and silver nitrate, and were irradiated with a low power (~ 8W) ultraviolet light for 6 hours. Their concentrations were idealized by the method of microplate dilution to determine the minimum inhibitory concentration when compared to the selected microorganisms. Bactericidal and fungicidal activities were identified with an initial concentration of 25% and a subsequent dilution factor of 12.5%.
Results: It was difficult to incorporate the AgNPs into the acrylic resin, which may well have resulted from the change from the 3:1 proportion recommended by the manufacturer or by reducing or inactivating the action of the silver nanoparticle by interaction with polymethylmethacrylate. VIPI with the inclusion of nanoparticles obtained a lesser Candida albicans biofilm adherence.
Conclusion: Silver nanoparticles were effective in controlling Candida albicans and Staphylococcus aureus in the immersion method; however, the antimicrobial activity was compromised after inclusion in acrylic resins.
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MB, Demarco FF. Uso e necessidade de prótese dentária em idosos brasileiros segundo a Pesquisa Nacional de Saúde Bucal (SBBrasil 2010): prevalências e fatores associados. Cad Saúde Pública. 2017;33(8):1-12.
Barbato PR, Nagano HCM, Zanchet FN, Boing AF, Peres MA. Perdas dentárias e fatores sociais, demográficos e de serviços associados em adultos brasileiros: uma análise dos dados do Estudo Epidemiológico Nacional (Projeto SB Brasil 2002-2003). Cad Saúde Pública. 2007;23(8):1803-14.
Camacho DP, Svidzinski TIE, Furlaneto MC, Lopes MB, Corrêa GO. Resinas acrílicas de uso odontológico à base de polimetilmetacrilato. Braz J Surg Clin Res. 2014;6(3):63-72.
Totu EE, Nechifor AC, Nechifor G. Aboul-Enein H. Poly(methyl metacrylate) TiO2 nanocomposite for stereolitographic complete denture manufacturing. J Dent. 2017;59:68-77.
Melo IA, Guerra RC. Oral candidiasis: a focus on denture stomatitis. Rev Salusvita. 2014;33(3):389-414.
Olms C, Doktor MY, Remmerbach TW, Stingu CS. Bacterial colonization and tissue compatibility of denture base resins. J Dent. 2018;6(2):20.
Nobrega DRM, Lucena AG, Medeiros LADM, Farias, TSS, Meira KRS, Mahon SMOD. Avaliação da utilização e hábitos de higiene em usuários de prótese dentária removível. Rev Bras Odontol. 2016;73(3):193-7.
Barreto JO, Silva FJA, Oliveira VC, Lovato CHS, Silva PG, Regis RR1. The effect of a continuous mechanical polishing protocol on surface roughness, biofilm adhesion, and color stability of acrylic resin artificial teeth. J Prosthodont. 2019;28(1):110-7.
Al-Fouzan AF, Al-Mejrad LA, Albarrag AM. Adherence of Candida to complete denture surfaces in vitro: a comparison of conventional and CAD/CAM complete dentures. J Adv Prosthodont. 2017;9:(5):402-8.
Garbacz K, Kwapisz E, Wierzbowska M. Denture stomatitis associated with smallcolony variants of Staphylococcus aureus: a case report. BMC Oral Health. 2019;19:219.
Kim JS, Kuk U, Yu Kn, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3(1):95-101.
Panacek A, Kvítek L, Prucek R, Kolář M, Večeřová R, Pizúrová N. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem, B. 2006;110(33):16248-53.
Köroğlu A, Sahin O, Kurkçuoglu I, Dede DO, Ozdemir T, Hazer B. Silver nanoparticle incorporation effect on mechanical and thermal properties of denture base acrylic resins. J Appl Oral Sci. 2016;24(6):590-6.
Torres ALS, Mendieta I, Anita NRE, Juárez CM, Castaño VM. Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures. Int J Nanomed. 2012;7:4777-86.
Wady AF, Machado AL, Zucolotto V, Zamperini CA, Berni E. Formation on a denture base acrylic resin containing silver nanoparticles. J Appl Microbiol. 2012;112(6):1163-72.
Castro DT, Valente MLC, Silva CHL, Watanabe E, Siqueira RL, Schiavon MA. Evaluation of antibiofilm and mechanical properties of new nanocomposites based on acrylic resins and silver vanadate nanoparticles. Arch Oral Biol. 2016;67:46-53.
Stieven E, Conde A, Galafassi D, Elsemann EB, Gazzoni AF. Análise da utilização de nanopartículas de prata e/ou plasma de baixa pressão e temperatura para a prevenção de candida albicans em próteses dentárias. Rev Bras Inov Tecnol Saúde. 2018;8(1):67-77.
Ostrosky EA, Mizumoto MK, Lima MEL, Kaneko TM, Nishikawa SO, Freitas BR. Métodos para avaliação da atividade antimicrobiana e determinação da concentração mínima inibitória (CMI) de plantas medicinais. Rev Bras Farmacogn. 2008;18(2):301-7.
Roca BM, Poestera VRB, Mattei ASC, Klafkea BC, Ramis IVB, Xaviera MO. Avaliação do uso da resazurina em teste de suscetibilidade in vitro frente a Sporothrix brasiliensis. Vittalle. 2019;31(2):32-7.
Souza Neto FN, Sala RL, Fernandes, RA, Xavier TPO, Cruz SA, Paranhos CM, et al. Effect of synthetic colloidal nanoparticles in acrylic resin of dental use. Eur Polym J. 2019;112:531-8.
Li Z, Sun J, Lan J, Qi Q. Effect of a denture base acrylic resin containing silver nanoparticles on Candida albicans adhesion and biofilm formation. Gerodontology. 2014;33(2):209-16.
Matteis V, Cascione M, Toma CC, Albanese G, Giorgi ML, Corsalini M, et al. Silver nanoparticles addition in poly(methyl methacrylate) dental matrix: topographic and antimycotic studies. Int J Mol Sci. 2019;20:4691.
Nam KY, Lee CH, Lee CJ. Antifungal and physical characteristics of modified denture base acrylic incorporated with silver nanoparticles. Gerodontology. 2012;29:413-9.
Di Martino A, Kucharczy P, Capakova Z, Humpolicek P, Sedlarik V. Chitosan-based nanocomplexes for simultaneous loading, burst reduction and controlled release of doxorubicin and 5-fluorouracil. Int J Biol Macromol. 2017;102:613-24.
Fan C, Chu L, Rawls HR, Norling BK, Cardenas HL, Whang K. Development of an antimicrobial resin – a pilot study. Dent Mater. 2011;27(4):322-8.
Kassaee MZ, Akhavan A, Sheikh N, Sodagar A. Antibacterial effects of new dental acrylic resin containing silver nanoparticles. J Appl Polym Sci. 2008;110(3):1699-1703.
Wady AF, Machado AL, Zucolotto V, Zamperini CA, Berni E, Vergani CE. Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles. J Appl Microbiol. 2012;112(6):1163-72.
Kumar R, Munstedt H. Silver ion release from antimicrobial polyamide silver composites. Biomaterials. 2005;26(4):2081-8.
Damm C, Munstedt H, Rosch A. Long-term antimicrobial polyamide 6 silver-nanocomposites. J Mater Sci. 2007;42:6067-73.
