Assessment of the quantity and purity of DNA obtained from buccal cells under different storage methods

Autores

  • Tayla Cavallari Pontifícia Universidade Católica do Paraná (PUC/PR)
  • Letícia Yumi Arima Pontifícia Universidade Católica do Paraná (PUC/PR)
  • Simone Tetu Moysés Pontifícia Universidade Católica do Paraná (PUC/PR)
  • Samuel Jorge Moysés Pontifícia Universidade Católica do Paraná (PUC/PR)
  • Renata Iani Werneck Pontifícia Universidade Católica do Paraná (PUC/PR)

Resumo

Aim: This study aimed to verify the quantity and purity of DNA obtained from buccal cells under differentstorage conditions. Methods: Thirty students, between 18 and 23 years of age participated in the study. Three samples of genetic material were collected from each student (samples A, B, and C) through a mouth rinse with 5 mL of 3% glucose. The first phase of DNA extraction from sample A was carried out on the same day of sample collection, whereas samples B and C were stored in a refrigerator and a freezer, respectively, for 1 month before the first extraction phase. DNA extraction was performed with 10 M ammonium acetate and 1 mM EDTA. Sample purity was assessed by spectrophotometry. Statistical analyses were performed through descriptive analysis and analysis of variance ANOVA using the SPSS software, version 21.0. Results: the samples presented no statistically significant differences between the DNA quantity (p = 0.37) or quality (p = 0.16). Conclusion: the quantity and purity of DNA extraction from the three samples were satisfactory, and no differences in storage conditions were found. Uniterms: DNA. Mouth. Mucosa.

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Biografia do Autor

Tayla Cavallari, Pontifícia Universidade Católica do Paraná (PUC/PR)

Mestre, Departamento de Pós-Graduação em Saúde Coletiva, Pontifícia Universidade Católica do Paraná (PUC/PR), Curitiba, Paraná, Brasil.

Letícia Yumi Arima, Pontifícia Universidade Católica do Paraná (PUC/PR)

Mestre, Departamento de Pós-Graduação em Saúde Coletiva, Pontifícia Universidade Católica do Paraná (PUC/PR), Curitiba, Paraná, Brasil.

Simone Tetu Moysés, Pontifícia Universidade Católica do Paraná (PUC/PR)

Doutor (a), Departamento de Pós-Graduação em Saúde Coletiva, Pontifícia Universidade Católica do Paraná (PUC/PR), Curitiba, Paraná, Brasil.

Samuel Jorge Moysés, Pontifícia Universidade Católica do Paraná (PUC/PR)

Doutor (a), Departamento de Pós-Graduação em Saúde Coletiva, Pontifícia Universidade Católica do Paraná (PUC/PR), Curitiba, Paraná, Brasil.

Renata Iani Werneck, Pontifícia Universidade Católica do Paraná (PUC/PR)

Doutor (a), Departamento de Pós-Graduação em Saúde Coletiva, Pontifícia Universidade Católica do Paraná (PUC/PR), Curitiba, Paraná, Brasil.

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Como Citar

Cavallari, T., Arima, L. Y., Moysés, S. T., Moysés, S. J., & Werneck, R. I. (2017). Assessment of the quantity and purity of DNA obtained from buccal cells under different storage methods. Arquivos Em Odontologia, 53. Recuperado de https://periodicos.ufmg.br/index.php/arquivosemodontologia/article/view/3735

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