Assessment of the quantity and purity of DNA obtained from buccal cells under different storage methods
DOI:
https://doi.org/10.7308/aodontol/2017.53.e17Keywords:
DNA, Mouth, MucosaAbstract
Aim: This study aimed to verify the quantity and purity of DNA obtained from buccal cells under different storage conditions.
Methods: Thirty students, between 18 and 23 years of age participated in the study. Three samples of genetic material were collected from each student (samples A, B, and C) through a mouth rinse with 5 mL of 3% glucose. The first phase of DNA extraction from sample A was carried out on the same day of sample collection, whereas samples B and C were stored in a refrigerator and a freezer, respectively, for 1 month before the first extraction phase. DNA extraction was performed with 10 M ammonium acetate and 1 mM EDTA. Sample purity was assessed by spectrophotometry. Statistical analyses were performed through descriptive analysis and analysis of variance ANOVA using the SPSS software, version 21.0.
Results: the samples presented no statistically significant differences between the DNA quantity (p = 0.37) or quality (p = 0.16).
Conclusion: the quantity and purity of DNA extraction from the three samples were satisfactory, and no differences in storage conditions were found.
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References
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