EFFECTS OF THE EGF AND THE TGF-ß ON THE DEVELOPMENT AND COMPONENTS OF THE EXTRA- CELLULAR MATRIX OF TOOTH GERMS IN VITRO
Keywords:
EGF, Tooth germs, Development, Connective matrix, Mouse, TGF-ßAbstract
Effects of the EGF and the TGF-b on the development and components of the extra-cellular matrix of tooth germs in vitro. Molar tooth germs of 14 or 17 days old mice fetuses were cultivated with EGF or TGF-b in order to study the effects of these growth factors on the tooth germ development and on the components of the connective tissue’s extra-cellular matrix. After the observation times, the tooth germs were embedded in paraffin, cut and stained with hematoxylin and eosin; or, frozen in liquid nitrogen, cut and submitted to immunocytochemical reaction for laminin, fibronectin or type I collagen. The results showed several effects, which allowed the following conclusions. 1. There was cell proliferation stimulation, delay of the tooth germ development, and inhibition of cell differentiation in the presence of EGF, and even more in the presence of TGF-b. 2. EGF and TGF-b did not interfere with the presence and distribution of the connective tissue’s extra-cellular matrix’s components. 3. Laminin was present in the enamel, dental papilla and dental follicle, gingival epithelium-ectomesenchyme interfaces, and in vascular structures of dental papilla and dental follicle. 4. Fibronectin was found in the dental papilla and dental follicle, particularly in tooth germs of older animals, and between the inner enamel epithelium and the dental papilla. 5. Type I collagen was found among the enamel organ epithelium, the dental papilla, and the dental follicle, as well as in specific sites of these structures.
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