Avaliação in vitro da citotoxicidade de dentes artificiais de diferentes marcas comerciais
DOI:
https://doi.org/10.7308/aodontol/2012.48.3.01Palavras-chave:
Dente artificial, Estudos de avaliação, Linhagem celularResumo
Objetivo: O objetivo deste estudo foi avaliar a citotoxicidade de diferentes marcas de dentes de estoque contra células fibroblásticas.
Materiais e Métodos: Para esta experiência a linhagem celular utilizada foi L 929 American Type Culture Collection (ATCC, Rockville, MD). As marcas de dentes de estoque testadas foram: Artplus, Biolux, Biotone, Pop Dent, Vipi Dent, Orthosit Ivoclar, Biocler Trilux. Cada grupo foi colocado em contacto com as células fibroblasticas nos períodos de 24, 48, 72 e 168 horas. Controles positivo e negativo foram usados para comparação com os extremos.
Resultados: Os resultados mostraram citotoxicidade para todos os grupos testados nos períodos de 24, 48 e 72 horas da realização do experimento, o que ocorreu para os Grupos Orthosit Ivoclar e Trilux ainda após 168 horas de condução do experimento.
Conclusão: Os dentes de acrílico estudados pesquisadas desenvolveram citotoxicidade contra as células fibroblásticas in vitro, nos períodos de 24, 48 e 72 horas e Vivodent Ivoclar e Trilux desenvolveram citotoxicidade contra as células testadas até o período de 168horas.
Downloads
Referências
Kiyak HA, Mulligan K. Studies of the relationship between oral health and psychological wellbeing. Gerodontics. 1987; 3:109-12.
Tang ATH, Li J, Ekstrand J, Liu Y. Cytotoxicity tests of in situ polymerized resins: methodological comparisons and introduction of a tissue culture insert as a testing deice. J Biomed Mater Res. 1999; 45:214-22.
Park JB. Biomaterials. In: Bronzino JD, editor. Biomedical engineering handbook. Boca Raton: CRC Press and IEEE Press; 1995. p.530–610.
Beall JR. Wear of acrylic resin teeth. J Am Dent Assoc. 1943; 30:252-56.
Appelbaum M. Theories of posterior tooth selection: porcelain versus acrylic. Dent Clin North Am. 1984; 28:299-306.
Craig RG, O’Brien WJ, Powers JM. Plásticos em Prótese. In: Craig RG, O’Brien WJ, Powers JM, editors. Materiais dentários: propriedades e manipulação. Rio de Janeiro: Guanabara Koogan; 1988. p.169-84.
Winkler S, Monasky GE, Kwok J. Laboratory wear investigation of resin posterior denture teeth. J Prosthet Dent. 1992; 67:812-4.
Hirano S, May KB, Wagner WC, Haker CH. In vitro wear of resin denture teeth. J Prosthet Dent. 1998; 2:152-5.
Kawano F, Ohguri T, Ichikawa T, Mizuno I, Hasegawa A. Shock absorbability and hardness of commercially available denture teeth. Int J Prosthodont. 2002; 15:243-7.
Neyndorff HC, Bartel DL, Tufaro F, Levy JG. Development of a model to demonstrate photosensitizer-mediated viral inactivation in blood. Transfusion. 1990; 30:485-90.
Anusavice, KJ. Phillips materiais dentários. 11nd ed. Rio de Janeiro: Elsevier; 2005.
Harrison A, Huggett R. Effect of the curing cycle on residual monomer levels of acrylic resin denture base polymers. J Dent. 1992; 20:370-4.
Lefebvre CA, Knoernschild KL, Schuster GS. Cytotoxicity of eluates from light-polymerized denture base resins. J Prosthet Dent. 1994; 72:644-50.
Schuster GS, Lefebvre CA, Dirksen TR, Knoernschild KL, Caughman GB. Relationships between denture base resin cytotoxicity and cell lipid metabolism. Int J Prosthodont. 1995; 8:580- 6.
Tsuchiya H, Hoshino Y, Tajima K, Takagi N. Leaching and cytotoxicity of formaldehyde and methyl methacrylate from acrylic resin denture base materials. J Prosthet Dent. 1994; 71:618-24.
Wennberg A. Biological evaluation of root canal antiseptics using in vitro and in vivo methods. Scand J Dent Res. 1980; 88:46-52.
Campanha NH, Pavarina AC, Giampaolo ET, Machado AL, Carlos IZ, Vergani CE. Cytotoxicity of hard chairside reline resins: effect of microwave irradiation and water bath postpolymerization treatments. Int J Prosthodont. 2006; 19:195-201.
Jorge JH, Giampaolo ET, Vergani CE, Pavarina AC, Machado AL, Carlos IZ. Effect of microwave postpolymerization treatment and of storage time in water on the cytotoxicity of denture base and reline acrylic resins. Quintessence Int. 2009; 40:93-100.
Shim JS, Watts DC. Residual monomer concentrations in denture-base acrylic resin after an additional, soft-liner, heat-cure cycle. Dent Mater. 1999; 15:296-300.
Sheridan PJ, Koka S, Ewoldsen NO, Lefebvre CA, Lavin MT. Cytotoxicity of denture base resins. Int J Prosthodont. 1997; 10:73-7.
Vallittu PK, Miettinen V, Alakuijala P. Residual monomer content and its release into water from denture base materials. Dent Mater. 1995; 11:338- 42.
Bagis YH, Rueggeberg FA. The effect of post- cure heating on residual, unreacted monomer in a commercial resin composite. Dent Mater. 2000; 16:244-7.
