Anti-microbial evaluation of peracetic acid as a disinfectant for dental impressions


  • Dimitri Ribas Fonseca Universidade Federal dos Vales do Jequitinhonha e Mucuri
  • Paulo Badaró de Souza Cirurgião-Dentista
  • Vitor César Dumont Universidade Federal dos Vales do Jequitinhonha e Mucuri
  • Paula Cristina Pelli Paiva Universidade Federal dos Vales do Jequitinhonha e Mucuri - UFVJM
  • Patrícia Furtado Gonçalves Universidade Federal dos Vales do Jequitinhonha e Mucuri
  • Maria Helena Santos Universidade Federal dos Vales do Jequitinhonha e MucuriM


Peracetic acid, Disinfection, Dental impression materials


Aim: This pilot study aimed to evaluate the disinfectant activity of peracetic acid at 0.2% in specimens of irreversible hydrocolloid.

Materials and Methods: This peracetic acid’s antimicrobial activity was investigated by comparing a 2% glutaraldehyde solution, a 2.5% sodium hypochlorite solution and   a   chlorhexidine   gluconate   added to the formula of an irreversible hydrocolloid. Four strains of microorganisms were selected: Escherichia coli, Staphylococcus aureus, Bacillus proteus, and Candida   albicans   for   inoculation in culture mediums. Irreversible hydrocolloid samples were randomly divided into five groups: G1 - with 8 samples of irreversible hydrocolloid without treatment disinfectant, representing the control group; G2 - with 8 samples of irreversible hydrocolloid disinfected by immersion in 2.5% sodium hypochlorite solution; G3 - with 8 samples of irreversible hydrocolloid disinfected by immersion in a 2% glutaraldehyde solution; G4 - with 8 samples of irreversible hydrocolloid disinfected by immersion in peracetic acid at 0.2%; G5 - with 8 samples of irreversible hydrocolloid with chlorhexidine gluconate added to the formula. Petri dishes containing fragments of irreversible hydrocolloid and seeded microorganisms were incubated for 24 h at 37°C. The inhibition of microbial growth ranged from 0 to 50 mm.

Results: The descriptive analysis showed antimicrobial activity of peracetic acid for all tested microorganisms. Intergroup analysis, performed in pairs with test group G4, by means of the Mann-Whitney test (p <0.05), showed no statistical significance. In assessing the magnitude of the Cohen effect, one can observe a similar behavior for the peracetic acid disinfectant, glutaraldehyde, and sodium hypochlorite, as well as a difference in the peracetic acid for the control groups G1 and G5 with chlorhexidine gluconate.

Conclusion: All strains were susceptible to peracetic acid, sodium hypochlorite, as well as Glutaraldehyde.


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How to Cite

Fonseca, D. R., Souza, P. B. de, Dumont, V. C., Paiva, P. C. P., Gonçalves, P. F., & Santos, M. H. (2016). Anti-microbial evaluation of peracetic acid as a disinfectant for dental impressions. Arquivos Em Odontologia, 47(3). Retrieved from




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