Anti-microbial evaluation of peracetic acid as a disinfectant for dental impressions
Keywords:
Peracetic acid, Disinfection, Dental impression materialsAbstract
Aim: This pilot study aimed to evaluate the disinfectant activity of peracetic acid at 0.2% in specimens of irreversible hydrocolloid.
Materials and Methods: This peracetic acid’s antimicrobial activity was investigated by comparing a 2% glutaraldehyde solution, a 2.5% sodium hypochlorite solution and a chlorhexidine gluconate added to the formula of an irreversible hydrocolloid. Four strains of microorganisms were selected: Escherichia coli, Staphylococcus aureus, Bacillus proteus, and Candida albicans for inoculation in culture mediums. Irreversible hydrocolloid samples were randomly divided into five groups: G1 - with 8 samples of irreversible hydrocolloid without treatment disinfectant, representing the control group; G2 - with 8 samples of irreversible hydrocolloid disinfected by immersion in 2.5% sodium hypochlorite solution; G3 - with 8 samples of irreversible hydrocolloid disinfected by immersion in a 2% glutaraldehyde solution; G4 - with 8 samples of irreversible hydrocolloid disinfected by immersion in peracetic acid at 0.2%; G5 - with 8 samples of irreversible hydrocolloid with chlorhexidine gluconate added to the formula. Petri dishes containing fragments of irreversible hydrocolloid and seeded microorganisms were incubated for 24 h at 37°C. The inhibition of microbial growth ranged from 0 to 50 mm.
Results: The descriptive analysis showed antimicrobial activity of peracetic acid for all tested microorganisms. Intergroup analysis, performed in pairs with test group G4, by means of the Mann-Whitney test (p <0.05), showed no statistical significance. In assessing the magnitude of the Cohen effect, one can observe a similar behavior for the peracetic acid disinfectant, glutaraldehyde, and sodium hypochlorite, as well as a difference in the peracetic acid for the control groups G1 and G5 with chlorhexidine gluconate.
Conclusion: All strains were susceptible to peracetic acid, sodium hypochlorite, as well as Glutaraldehyde.
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