In vitro study of the effect of 17% EDTA application at different times in the smear layer removal in the apical third of root canals
Keywords:
Chelanting agents, EDTA, Biomechanical preparation, Smear layerAbstract
The aim of this study was to evaluate the effect of the application of 17% EDTA at different times in the removal of the smear layer of the apical third of root canals. Twenty human maxillary central incisors were divided into two groups (n =10). In Group I (GI), the instrumentation of the apical third was performed using K files in the step-back technique, with an irrigation of 1ml of a 2.5% sodium hypochlorite solution during each instrument change, and a final irrigation of 1ml of a 17% EDTA solution for 5 minutes. Group II (GII) underwent the same instrumentation procedure but was irrigated with 1ml of a 17% EDTA solution during each instrument change. After, the specimens were split longitudinally for evaluation via scanning electronic microscopy (SEM). The photomicrographs obtained were analyzed, and the amount of smear layer was measured following a pre-established score (1 - without smear layer, 2 - partial removal of the smear layer with dentinal tubules partially open, 3 - smear layer partially present in the dentinal surface with obliterated tubules, and 4 - all of the walls covered by smear layer). The data were submitted to statistical analysis by means of the Mann-Whitney test (p£0.05). The results demonstrated that no significant statistical difference among the techniques of application of EDTA could be observed either during or after instrumentation. It could therefore be concluded that both techniques were capable of removing the smear layer from the walls of the apical third of the researched specimens, with no statistical difference among them.
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