Efeito antimicrobiano in vitro do Agregado Trióxido Mineral (MTA) sobre bactérias Gram-negativas e Gram-positivas
DOI:
https://doi.org/10.35699/2178-1990.2026.63330Palavras-chave:
cimentos dentários, antibacterianos, testes de sensibilidade microbiana, Staphylococcus aureus, Enterococcus faecalis, cimento de silicatoResumo
Introdução: o Agregado Trióxido Mineral (MTA) é amplamente empregado na Endodontia por suas propriedades biocompatíveis e potencial antimicrobiano. No entanto, a influência do estado físico do material sobre sua atividade antibacteriana ainda carece de investigação padronizada.
Objetivo: Avaliar, in vitro, a atividade antimicrobiana do MTA branco e cinza em diferentes formas de apresentação — pó, recém-manipulado e pós-presa — frente a bactérias Gram-positivas e Gram-negativas.
Materiais e Métodos: utilizou-se o método de difusão em ágar (Mueller-Hinton) para avaliação da atividade antimicrobiana do MTA branco e cinza (Angelus Soluções Odontológicas, Londrina, Brasil) frente a culturas puras de Staphylococcus aureus, Streptococcus mutans, Streptococcus sobrinus, Enterococcus faecalis, Escherichia coli e Pseudomonas aeruginosa. Os resultados foram submetidos à ANOVA seguida do teste de Tukey (α=5%).
Resultados: o MTA cinza em pó apresentou os maiores halos de inibição (até 14,33 mm para S. aureus), enquanto as formas recém-manipuladas exibiram os menores valores. Bactérias Gram-negativas demonstraram menor sensibilidade em relação às Gram-positivas.
Conclusões: o estado físico do MTA influencia diretamente sua atividade antimicrobiana in vitro. Os achados devem ser interpretados com cautela, dado o delineamento experimental in vitro, que não reproduz integralmente as condições clínicas dos canais radiculares.
Referências
Velickovic S, Pavlovic S, Markovic BS, Jovanovic I, Zornic S, Kragovic M, et al. Evaluation of biocompatibility, antimicrobial activity, and immunomodulatory effects of mineral trioxide aggregate modified with zeolite-clinoptilolite as a root-end filling material. BMC Oral Health. 2025;25:1159. doi: 10.1186/s12903-025-06587-x
Moon S, Shin S, Oh S, Bae J. Antibacterial activity and sustained effectiveness of calcium silicate-based cement as a root-end filling material against Enterococcus faecalis. Materials (Basel). 2023;16(18):6124. doi: 10.3390/ma16186124
Wang X, Xiao Y, Song W, Ye L, Yang C, Xing Y, et al. Clinical application of calcium silicate-based bioceramics in endodontics. J Transl Med. 2023;21:853. doi: 10.1186/s12967-023-04550-4
Ravindran V, Jeevanandan G. Comparative evaluation of the physical and antimicrobial properties of mineral trioxide aggregate, biodentine, and a modified fast-setting mineral trioxide aggregate without tricalcium aluminate: an in vitro study. Cureus. 2023;15(8):e42856. doi: 10.7759/cureus.42856
Acharya S, Gurunathan D, Sahoo D, Singh B, Sahoo A, Acharya S. Comparative evaluation of the antimicrobial activity of NeoPutty MTA and modified NeoPutty MTA: an in vitro study. J Int Soc Prev Community Dent. 2023;13(6):493-9. doi: 10.4103/jispcd.JISPCD_68_23
Bloch S, Hager-Mair FF, Andrukhov O, Schäffer C. Oral streptococci: modulators of health and disease. Front Cell Infect Microbiol. 2024;14:1357631. doi: 10.3389/fcimb.2024.1357631
Deng Z, Lin B, Liu F, Zhao W. Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response. J Oral Microbiol. 2023;15:2184924. doi: 0.1080/20002297.2023.2184924
Korona-Glowniak I, Piatek D, Fornal E, Lukowiak A, Gerasymchuk Y, Kedziora A, et al. Patterns of oral microbiota in patients with apical periodontitis. J Clin Med. 2021;10(12):2707. doi: 10.3390/jcm10122707
Kesim B, Ülger ST, Aslan G, Cudal H, Üstün Y, Küçük MÖ. Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections. Clin Oral Investig. 2023;27(3):995-1004. doi: 10.1007/s00784-023-04882-x
Mehrabkhani M, Movahhed T, Arefnezhad M, Hamedi S, Faramarzian F. Antimicrobial effect of hydro-alcoholic extract of apple with and without zinc oxide nanoparticles on Streptococcus mutans. Eur J Transl Myol. 2023;33(4):11623. doi: 10.4081/ejtm.2023.11623
Meire MA, van der Waal SV. A critical analysis of research methods and experimental models to study intracanal medicaments. Int Endod J. 2022;55 Suppl 2:330-45. doi: 10.1111/iej.13694
Jiang W, Zhang Y, Yan J, He Z, Chen W. Differences of protein expression in Enterococcus faecalis biofilm during resistance to environmental pressures. Technol Health Care. 2024;32(1 Suppl):371-83. doi: 10.3233/THC-248033
Liu C, Li Y, Li Y, Li Z, Han G. Synergistic antibacterial effect of ginsenoside Rh2 and calcium hydroxide on Enterococcus faecalis. Odontology. 2025;113:111-25. doi: 10.1007/s10266-024-00951-z
Ali IA, Cheung GS, Neelakantan P. Transition metals and Enterococcus faecalis: homeostasis, virulence and perspectives. Mol Oral Microbiol. 2022;37(6):276-91. doi: 10.1111/omi.12391
Chakravorty A, Ravindran V, Jeevanandan G, Arthanari A. The cytotoxic assessment of antibacterial-enhanced mineral trioxide aggregate compared to commercially available bioceramic cements by using methyl-thiazoldiphenyl-tetrazolium (MTT) assay on human dental pulp stem cells: an in vitro study. Cureus. 2023;15(11):e49691. doi: 10.7759/cureus.49691
ElReash AA, Hamama H, Eldars W, Lingwei G, Zaen El-Din AM, Xiaoli X. Antimicrobial activity and pH measurement of calcium silicate cements versus new bioactive resin composite restorative material. BMC Oral Health. 2019;19:235. doi: 10.1186/s12903-019-0933-z
Chaudhari PS, Chandak MG, Jaiswal AA, Mankar NP, Paul P. A breakthrough in the era of calcium silicate-based cements: a critical review. Cureus. 2022;14(8): e28562. doi: 10.7759/cureus.28562
