Artificial insemination and gestation follow-up in Bull Terrier dog breed with aggressiveness’s history
case report
DOI:
https://doi.org/10.35699/2447-6218.2020.16292Keywords:
Canine reproduction, Vaginal citology, Estrus CycleAbstract
The objective of this work was to report an artificial insemination (AI) and subsequent gestational follow - up performed on a Bull Terrier bitch with a history of aggressiveness. A 14-month-old Bull Terrier was received with apparent signs of estrus, which showed aggression and intolerance to the approach of the male in the natural mating, and AI was suggested. Vaginal cytology confirmed the estrus phase, with more than 80% of anucleated keratinized cells. The semen of the male donor was collected by digital manipulation and later analyzed, presenting quality in all sperm parameters evaluated. Intravaginal artificial insemination was performed with fresh semen, and the same procedure was repeated 48 hours after a second vaginal cytology confirming that the animal was still in estrus. This repetition of AI was designed to better cover the duration of estrus, thus increasing the chance of breeding. After 51 days of the last AI, an ultrasonography was performed to confirm gestation. It was possible to observe the presence of live fetuses, whose viability was confirmed by the presence of heart beats between 230 and 257 bpm. The birthing occurred 58 days after the last insemination, lasting approximately eight hours and birth of five pups, all alive and healthy. It was possible to conclude that AI after confirmation of estrus by vaginal cytology and gestational monitoring are important tools of reproduction biotechnology capable of providing the use of animals in reproductive activity in cases where natural reproduction is not possible.
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References
Castro, V. M. et al. 2011. Acompanhamento da Gestação em Cadelas pelo exame Ultrassonográfico. Revisão de Literatura. Veterinária e Zootecnia, 18(1): 9–18. Disponível em: https://repositorio.unesp.br/handle/11449/141240.
Colégio Brasileiro de Reprodução Animal - CBRA. 2013. Manual para Exame Andrológico e Avaliação do Sêmen Animal. 3 ed. Belo Horizonte, Brasil.
Costa, E. C. F. et al. 2009. Estimativa da fase do ciclo estral por citologia vaginal em cadelas (canis familiaris, linnaeus, 1758) da região de Ituverava-sp. Nucleus Animalium, 1(2). Doi: 10.3738/1982.2278.310.
Dar, R. R. et al. 2017. Exfoliative Vaginal Cytology (EVC) as a tool for determining breeding time in the bitch a case study. Journal of Entomology and Zoology Studies, 5(6):1446-1448. Disponível em: http://www.entomoljournal.com/archives/2017/vol5issue6/PartT/5-6-140-949.pdf.
Davidson, A. P.; Baker, T. W. 2009. Reproductive ultrasound of the Bitch and Queen. Topics in Companion Animal Medicine, 24(2): 55-63. Doi: https://doi.org/10.1053/j.tcam.2008.11.002.
Duffy, D. L. et al. 2008. Breed differences in canine aggression. Applied Animal Behaviour Science, 114: 441–460. Doi: 10.1016/j.applanim.2008.04.006.
England, G. C. W. et al. 2010. Heritability of semen characteristics in dogs. Theriogenology, 74(7): 1136–1140. Doi: http://dx.doi.org/10.1016/j.theriogenology.2010.05.012.
Feldman, E. C.; Nelson, R. W. 1996. Breeding, Pregnancy, and Parturition. p. 547-571. In: FELDMAN, E.C.; NELSON, R.W. Canine and feline endocrinology and reproduction. Saunders Company, Philadelphia.
Freitas, J. G.; Silva, A. R. 2008. Diagnóstico de gestação em cadelas. Revista Brasileira de Reprodução Animal, 32(1): 58-66. Disponível em: http://www.cbra.org.br/pages/publicacoes/rbra/download/RB144%20pag58.pdf.
Fukushima, F. B.et al. 2010. Site of Intrauterine Artificial Insemination in the Bitch does not Affect Sperm Distribution within the Uterus. Reproduction in Domestic Animals, 45: 1059–1064. Doi: 10.1111/j.1439-0531.2009.01493.x.
Karger S. et al. 2016. Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen. Animal Reproduction Science, 166: 141-147. Doi: http://dx.doi.org/10.1016/j.anireprosci.2016.01.015.
Lamm, C. G.; Makloski, C. L. 2012. Current advances in gestation and parturition in cats and dogs. Veterinary Clinics of North America: Small Animal Practice, 42(3): 445-456. Disponível em: https://www.vetsmall.theclinics.com/article/S0195-5616(12)00011-3/abstract.
Lopate, C. 2008. Estimation of gestational age and assessment of canine fetal maturation using radiology and ultrasonography: a review. Theriogenology, 70(3): 397-402. Doi: https://doi.org/10.1016/j.theriogenology.2008.05.034.
Mason, S. J. 2018. Current review of artificial insemination in dogs. Veterinary Clinic Small Animals, 48(4): 567-580. Doi: https://doi.org/10.1016/j.cvsm.2018.02.005.
Setyawan, E. M. N., et al. 2016. Spermine reduces reactive oxygen species levels and decreases cryocapacitation in canine sperm cryopreservation. Biochemical and Biophysical Research Communication, 479(4):927–32. Doi: http://dx.doi.org/10.1016/j.bbrc.2016.08.091.
Silva, A. R. et al. 2001. Criopreservação do sêmen canino: revisão. Ciência Animal, 11(2): 119-129. Disponível em: http://www.uece.br/cienciaanimal/dmdocuments/Artigo7.2001.2.pdf.
Silva, A. R. et al. 2003. Principais aspectos ligados à aplicação da inseminação artificial na espécie canina. Revista Portuguesa de Ciências Veterinárias, 98: 53-60. Disponível em: http://www.spcv.pt/download/Vol98/Vol98-n546.pdf.
Tesi, M. et al. 2018. Variables affecting semen quality and its relation to fertility in the dog: A retrospective study. Theriogenology, 118:34–39. Doi: https://doi.org/10.1016/j.theriogenology.2018.05.018.
Uchoa, D. C. S. et al. 2012. Criopreservação de sêmen e inseminação artificial em cães. Ciência Animal, 22 (1). Disponível em: http://www.uece.br/cienciaanimal/dmdocuments/CONERA_PALESTRA%20(10).pdf.
Veiga, C. C. P. et al. 2013. Avaliação pré-natal ultrassonográfica e radiográfica no diagnóstico de anencefalia em cão – relato de caso. Revista Brasileira de Medicina Veterinária, 35(2): 101-104. Disponível em: http://rbmv.org/index.php/BJVM/article/download/596/460.
Vieira, M. M. F. et al. 2012. Detecção do ciclo estral por meio de citologia vaginal de cadelas atendidas no hospital veterinário da Univiçosa/Facisa. Anais do IV SIMPAC, Viçosa, MG, Brasil. Disponível em: https://academico.univicosa.com.br/revista/index.php/RevistaSimpac/article/download/207/369.
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