In vitro control of broad bean Colletotrichum truncatum (Phaseolus lunatus) by Trichoderma spp.
DOI:
https://doi.org/10.35699/2447-6218.2020.18464Keywords:
Antagonism, Anthracnose, Biological control, Phaseolus lunatusAbstract
Anthracnose, caused by Colletotrichum truncatum is one of the main threats to the production of fava beans, therefore, control measures must be taken, aiming to minimize the problems caused by this disease, such as the use of biological control using Trichoderma spp.. This study aimed to evaluate the antagonistic potential of the strains of Trichoderma spp. on Colletotrichum truncatum, in vitro. (TCH01, TCH02, TCH03, TCH04 and TCH05) in antagonism to C. truncatum. In order to test the antagonistic potential of Trichoderma isolates on the pathogen, the direct confrontation test was performed using the paired culture methodology, in which the classification of the cluster of the antagonist isolates and microscopic examinations looking for signs of mycoparasitism were evaluated. The antibiosis test was also carried out, in which the measurement of colony diameter, percentage of mycelial growth inhibition, mycelial growth rate, spore count and percentage of sporulation inhibition were evaluated. By the direct confrontation test, all isolates of Trichoderma spp. were grouped in class 1, that is, they grew on the pathogen colony, occupying the entire plate. It was also observed that the antagonists have potential against C. truncatum in the antibiosis test, with mycelial growth inhibition and sporulation ranging from 74.7% to 82.0% and 78.7% to 89.3%, respectively. All the isolates evaluated were efficient antagonists for Colletotrichum truncatum.
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