Biofilm formation by multiresistants Staphylococcus aureus isolated from milk from cows with mastitis

Authors

  • Edmara Andrade Macedo Cruz Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-2493-978X
  • Ester Dias Xavier Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0001-8289-3540
  • Geziella Aurea Aparecida Damasceno Souza Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-7130-3776
  • Cintya Neves Souza Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-3640-8636
  • Carolina Magalhães Caires Carvalho Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-3145-0253
  • Daniele Pereira de Souza Borborema Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-7376-586X
  • Franciane Gabrielle Santos Universidade Federal de Minas Gerais, Instituto de Ciências Agrárias, Montes Claros, MG, Brasil. https://orcid.org/0000-0002-7552-3266
  • Raphael Rodrigues Porto Universidade Federal de Uberlândia, Patos de Minas, MG, Brasil. https://orcid.org/0000-0003-1603-9615

DOI:

https://doi.org/10.35699/2447-6218.2021.35651

Keywords:

Congo Red Agar, Microplate Adherence, Antimicrobials

Abstract

Staphylococcus aureus strains from the milk of cows with subclinical mastitis and phenotypically resistant to antimicrobials of the beta-lactam class were tested for capacity to produce biofilm. Was carried out cultivation and analysis in Congo Red Agar (CRA) was carried out and Plate Adhesion Test (AEP). The association between CRA and AEP and the association of each test with the frequency of potentially biofilm-producing strains resistant to beta-lactams was also evaluated. As a result, 65.2% (15) of the 23 isolates tested positive for biofilm formation in CRA and 60.86% (14) in AEP. There was no association between the results of CRA and AEP tests, nor was there an association with the beta-lactams antimicrobials resistance (RBL) test. There was an association between the AEP and RBL tests. It is considered that the ability to form biofilms is a powerful resistance mechanism and emphasizes the importance of microbiological control in production

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Published

2021-10-17

Issue

Section

Scientific Reports

How to Cite

Biofilm formation by multiresistants Staphylococcus aureus isolated from milk from cows with mastitis. (2021). Agrarian Sciences Journal, 13, 1-6. https://doi.org/10.35699/2447-6218.2021.35651
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